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Uptake of CLF To measure CLF polarity, organoids were removed from the Matrigel by washing with PBS and incubated with culture medium supplemented with 10 g/ml CLF (Corning Inc.) and 1 g/ml Hoechst 33342 for 30 minutes at 37 C in a 5% CO 2 incubator
The AUC of LC and ALC were 0.801 and 0.898, respectively
Nucleic Acids Res, 2012
Participants who had taken GLP-1s for 2 or 2.5 years before discontinuing use for the remainder of the study also saw a significant reduction in risk of 7% and 15%, respectively
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