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Cells were collected and washed with PBS once, and cellular fluorescence was determined by flow cytometry and data were collected by BD FACSCanto and analyzed with FlowJo V.10.1 software
Cysteine is a sulfur amino acid, so in those who can tolerate sulfur foods, consuming sulfur-rich foods, especially those containing the sulfur amino acids, may also support glutathione synthesis
Annu Rev Physiol 2013
Cell line sources Human skin fibroblasts (HSF, catalog number: PCS-201-012), mouse monocyte macrophage leukemia cells (RAW264.7, catalog number: TIB-71), human umbilical vein endothelial cells (HUVEC, catalog number: PCS-100-013) and human immortalized keratinocytes (HaCaT, catalog number: PCS-200-011) were obtained from the American Type Culture Collections (ATCC)
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