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Athletes assume responsibility for supplement use, and WADA recommends using reputable third-party certification programs if athletes choose to use dietary supplements

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They are produced in the heart, lungs, kidneys, and brain

After quantifying the concentrations of melatonin, serotonin, dopamine, glutamate, and neurotrophic factors using HPLC and ELISA, the results are analyzed using statistical software such as GraphPad Prism

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