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Zhang Y, Lu T, Han L, Zhao L, Niu Y, Chen H (2017) L-glutamine supplementation alleviates constipation during late gestation of mini sows by modifying the microbiota composition in feces
The side effects are similar to semaglutide GLP-1, primarily gastrointestinal discomfort
Proteomics 2017;17(20)
On the next day, targeting plasmids (sgRNA or overexpression) were co-transfected with pMD2.G (#12259, Addgene) and psPAX2 (#12260, Addgene) plasmids at 1:1:1 molar ratio using Lipofectamine 2000 (#11668019, Thermo Fisher) at 1:2.5 ratio
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