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Various factors are involved in the fluctuations and stabilities in bacterial levels, such as nutrient availability, temperature, osmolality, pH, and bacteriophages, which are viruses that infect bacteria 20,21,22,23
Thus, the TCA cycle can convert four molecules of NAD + to NADH using one molecule of pyruvate in the mitochondria under aerobic conditions
The reaction was then quenched by the addition of 5x reducing buffer (50% glycerol, 250 mM Tris-Cl [pH 6.9], 10% SDS, 0.05% bromophenol blue, 6.25% beta-mercaptoethanol), boiled and separated by sodium dodecyl sulfate polyacrylamide (SDS-PAGE) on 15% polyacrylamide gels
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