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Heliyon 10 , e32770 (2024)

As the FRET only occurs when the distance between donor and acceptor is less than 10 nm, it is useful for monitoring interactions between two fluorophore-fused molecules

For example, the delineation of sub-cellular folate and one-carbon metabolism in mitochondria and the nucleus (8), the importance of formate in said metabolism (9, 10), identification of the transcobalamin receptor by which vitamin B12 is taken up into cells (11), and delineation of the sequence of events underlying intracellular vitamin B12 processing (1214)

When elevated B12 is detected, confirmation with repeat testing is advisable

Yes, this idea freaks many patients out