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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )
Published preclinical work has examined BPC-157 in rat tendon explants and cultured fibroblasts
Paired-end reads were assembled by using PEAR v0.9.8 [32]
The specific strategy to target FcRn has proven to yield robust efficacy with minimal adverse effects for several autoimmune diseases and offers hope in both prevalent diseases, such as RA, and rare diseases with no or limited efficacious options, such as Sjgrens disease and HDFN
Results can last between 6 months to more with a healthy lifestyle
