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This can also affect vitamin B12 absorption
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"My hair is fuller, my skin is better, I have more energy, I want to get down more, if you know what I mean," she noted
The column was washed with 150 mL of the equilibration buffer and the protein was eluted using a 400 mL gradient from 10300 mM imidazole in 50 mM KPi, 100 mM NaCl, pH 7.4
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