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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min

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The use of peptides that have not been evaluated and approved by the FDA, or the use of FDA-approved peptides for purposes other than their specified indication, is known as 'off-label' use