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Researchers eventually renamed it glucose-dependent insulinotropic polypeptide , which more accurately reflects its role in regulating blood sugar after meals

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The adsorbed glycoproteins were eluted at 37 C for 30 min with sample buffer (400 mM Tris-HCl, 10% SDS, 0.5 M 2-mercaptoethanol, 50% glycerol, and 0.025% Bromophenolblau) and resolved on a 10% SDS-polyacrylamide gel