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IUBMB Life 59, 5159

(Lausanne) 14:1070264

Thus, the most promising quantitative approaches are those that rely on trapping the reactive sulfur atom(s) in a diagnostic product and then quantifying these, typically by mass spectrometry (MS) 28,31,32,33

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ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well