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The authors concluded that such a programmable and stimuli-responsive nanohydrogel can be explored further as a cancer therapeutic (Li et al., 2019), however, this study was confined to the in vitro level and its execution to an in vivo level necessitates additional studies, such as the suitable route of administration, immunogenicity, general toxicity, metabolic stability, and suitable pharmacokinetic profile

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Phenotypic Screening Deconvolution After a phenotypic screen identifies active compounds, metabolic fingerprinting provides the first mechanistic triage layer separating compounds that act through energy metabolism, membrane disruption, nucleotide biosynthesis, or signalling pathway inhibition without requiring individual target binding assays