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Avoiding repeated freeze-thaw cycles and storing samples at minus 80 degrees Celsius further preserves the native redox state of the analyte
25(OH)VD, insulin, glucose, GSH, protein carbonyl, and Malondialdehyde (MDA) assays Plasma levels of 25(OH)vitamin D were determined using an ELISA kit (Calbiotech, Spring Valley, CA), levels of insulin were determined using ELISA kits from ALPCO Diagnostics (Salem, NH)
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The aim of the present study was to investigate the therapeutic effect of LC in an animal model of PCOS induced by estradiol valerate (EV)
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