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The cells were subsequently received different treatments for 24 h and were cultured in a serum-free medium containing a labeling media, which consisted of BODIPY cholesterol (0.025 mM) (BODIPY-Cholesterol, Avanti Polar Lipids, Alabaster, AL, United States), MCD (10 mM, sigma), HEPES (Sigma), and egg phosphatidylcholine (0.1 mM) (Avanti Polar Lipids) (Sankaranarayanan et al., 2011) for 1 h

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Molecular dynamics with coupling to an external bath

Most protocols separate Retatrutide from healing peptides by at least several hours, though no direct interaction mechanism exists