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To determine whether Trim37 -deletion-induced PGC depletion is germ cell autonomous, we first constructed a Trim37 knockout embryonic stem cell line ( Trim37 -del) based on an E14-derived ESC line in which 3Flag was knocked in at the C-terminus of Trim37 ( Trim37-3 Flag ) (Supplementary information, Fig
Higher concentrations (3%+) are best reserved for experienced users or targeted use, as they raise the risk of irritation and staining without clearly proportional benefits
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Human healing timelines are generally longer than animal models
This is why combining the two pathways produces additive effects
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