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The supernatants were removed and selected through a gravity flow nickel column containing 1 mL of NTA Ni Agarose (Qiagen)

For example, long-term starvation induces metabolic depression 102 , which compromises other fitness-related functions and lowers the animals capacity to cope with other stressors 28 , and hence metabolic adaptation is required to ensure survival

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Our injection guide covers proper subcutaneous technique