The strongest human evidence is topical
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At the respective analysis point, the medium was removed, washed with 200 l PBS and replenished with 200 l fresh medium per well
One large observational study linked low IGF-1 levels to higher body mass index (BMI) but couldnt prove cause and effect (15, 16, 17)
At the same time, the blends complexity demands rigorous experimental design, clear controls, and a constant awareness that all work remains within the research-only domain