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For mitochondrial proteomics, mitochondria were rapidly immunopurified from HeLa cells expressing a mitotag (3xHA-OMP25-mCherry) grown in indicated cell culture conditions, according to the rapid mitochondrial purification for metabolite profiling protocol
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The most commonly used models are LPC injection and CPZ dietary administration 3,4
a preclinical research compound
[PMID: 7080782] PubMed record related to this compound for laboratory literature review
Thereafter, mice were divided into two groups ( n = 6/group) and started on one of two treatments: a control diet (Con) and HFD
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