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IGF-1 (Insulin-like Growth Factor 1) is the downstream mediator responsible for most of GHs anabolic and lipolytic effects
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Trichoplusia ni cell pellets were suspended in 30 mM HEPES (pH 7.4), 50 mM NaCl, 5 mM CaCl 2 , 2 mM MgCl 2 supplemented with protease inhibitors (REF118361 45001, Roche) and benzonase (Merk Millipore)
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The freeze-drying process specifically makes them resistant to temperature fluctuations
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