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2c was obtained after a sample (from the control group) 5-fold pre-concentration (by solvent evaporation under reduced pressure), thus in the 36 min time range some peaks appear due to bilirubin degradation during sample concentration
When they do occur, they are typically mild and temporary
This could be particularly beneficial for active individuals and athletes
Only pharmaceutical-grade sterile or bacteriostatic water is acceptable
The absorbance (OD value) of each well was measured sequentially at a wavelength of 450 nm, with the blank well serving as the reference
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