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In in vivo studies, subcutaneous injection frequency, injection site rotation, and peptide stability in the dosing vehicle all represent variables that researchers must account for when designing replicable experimental protocols

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Dose-dependency of efficacy correlates with autophagy induction To evaluate whether effective antibiotic concentrations against Wolbachia also activate autophagy, and whether a dose-dependent effect could be seen, we measured each drugs anti- Wolbachia activity alongside autophagy induction

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