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Differential expression analysis Differential expression analysis was performed with DESeq2 using the Wald test with the fraction of ribosomal RNA included as a covariate

Melanocortins in research: complete guide to Melanotan II, PT-141 bremelanotide, afamelanotide and MC1R-MC5R receptors The melanocortin system is a central and peripheral neuroendocrine network formed by five G protein-coupled receptors (MC1R through MC5R), their endogenous ligands derived from proopiomelanocortin (POMC) alpha-MSH, beta-MSH, gamma-MSH, ACTH and their natural antagonists agouti (ASIP) and AgRP

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By activating intestinal hormone receptors, it regulates insulin secretion, delays gastric emptying, and suppresses appetite to achieve glycemic control and weight management