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Harvested cells were resuspended in 20 mM HEPES pH 7.0, 500 mM NaCl, 25 mM imidazole (lysis buffer) supplemented with protease inhibitor cocktail and then lysed by a cell disruptor at 25 kpsi (Constant Systems)
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Evidence is anecdotal rather than scientific, but its generally safe to try
GLP-1 may modulate lipid metabolism through increased sympathetic tone, leading to increased lipolysis, heightened triglyceride catabolism, and enhanced fatty acid oxidation (181)
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