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These pooled samples were treated as a biological sample and analyzed amongst the analytical batches to assess instrument repeatability and potential batch effects in the data

Adiponectin levels and genotype: a potential regulator of life span in humans

To move beyond basic cell culture, the study incorporated ex vivo skin models

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At specified time intervals (0 h, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, 48 h), the sample solution (1.5 mL) was withdrawn from the dialysate, after which an equal volume of fresh corresponding solution (1.5 mL) was promptly added back into the dialysate to maintain the overall volume