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Set baseline hormone tests, including total and free testosterone, LH, FSH, prolactin, estradiol, GH, and IGF-1, and repeat every planned interval to catch suppression or excess

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If long-term frozen storage is required, divide into single-use aliquots first and thaw each once

To harvest oocytes, an ultrasound-guided needle aspiration of the ovary is generally performed through the vaginal wall after ovulation stimulation, usually with an injection of gonadotropins