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Elegant 13 C-NMR analysis ( in vivo injection of labeled glucose, or in some cases acetate, and determination of labeled metabolites) has shown that the glutamine flux in the cycle, V gln in the 13 C-NMR studies, is slightly greater than the flux, V cyc in the 13 C-NMR studies, of released transmitter glutamate and GABA in the opposite direction (Rothman et al., 2011), and that GABA fluxes account for up to 20% of total flux in V cyc (Patel et al., 2005

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The application technique, frequency of use, combination with other active ingredients and number of sessions must be adapted to the professional assessment of each case and to the aesthetic response observed

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