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a , Relative metabolite abundance of GSSG and GSH in whole cell lysate (WC) and isolated ER fractions from HEK293T cells treated with or without 1 mM BSO for 48 h

doi:10.1016/j.mam.2008.08.006 2

During the synthesis process, the reaction conditions such as temperature, pH value and reagent concentration need to be precisely controlled

Two key enzymes, acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3), are involved in the biosynthesis and remodeling of PEs, activation of PUFAs, and insertion of PUFAs into membrane PLs (Doll et al., 2017)

The specific primers were designed to target the regions flanking the GR gene insertion site, ensuring the accurate identification of successful knockouts