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Sections of 4 m thickness are prepared using a paraffin microtome (HS3315, Huadu Technology, Zhejiang) and then dried in an oven (GZX-9030MBE, Boxun, Shanghai) at 60C for 1 h

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Figure 1 2.1 Overproduction of ROS One hallmark feature of IBD is the overproduction of ROS, which causes dysregulation of signal transduction, an inflammatory response, and DNA damage, all of which contribute to the progression and deterioration of the disease ( 2), nitric oxide (NO), hydroxyl radical (-0H), hydroperoxyl radical (O 2 H), hydrogen peroxide (H 2 O 2 ), and singlet oxygen ( 2.2 Damage to biomolecules ROS can damage various cell biomolecules, including lipids, proteins, and DNA

The thyroid is one of the most metabolically active organs in the human body