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ACTL6A impacts GSH de novo synthesis mainly by upregulating -glutamyl-cysteine synthesis To further assess the mechanism by which ACTL6A regulates GSH metabolism, we investigated the metabolic fate of U- 13 C glucose by liquid chromatography with mass spectrometry (LCMS), which produces -GC containing two 13 C atoms via GSH de novo synthesis pathway, GSH containing two 13 C atoms via GSH de novo synthesis or the SGOC pathway, or four 13 C atoms via both pathways (Fig

When tirzepatide binds to these receptors, it enhances glucose-dependent insulin secretion from pancreatic beta cells, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying

Transfer the supernatant to a new tube, and use it for the assay

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