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GHRP-6 maintains pulsatile release patterns and preserves feedback loops

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GST pull-down assay His-tagged purified proteins (200 ng/sample) were mixed with 100 ng of GST fusion proteins or GST protein as a control in a binding buffer (50 mM Tris-HCl [pH 7.5], 1% Triton X-100, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, 100 M PMSF, 100 M leupeptin) for 1 h and incubated with glutathione agarose beads for an additional 30 min at 4 C

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Anchor your dose to whichever compound matches your goal GHK-Cu for skincare, BPC-157 for injury repair and the other three peptides come along at fixed proportions