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Nevertheless, compounds that stimulate mitochondrial oxidative metabolism, including thyroid hormone receptor agonists20, ligands for nuclear receptors that control the expression of mitochondrial genes21 and inhibitors of mitochondrial pyruvate import22, have shown efficacy in the treatment of NASH

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Western blotting Cell lysates were collected in RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulphate)and Western blotting was performed as previously described 49

Deposition of ectopic fat in insulin-responsive metabolic tissues generally has an inhibitory effect on insulin signaling, a phenomenon commonly referred to as lipotoxicity