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According to the instructions of the Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062), 5 L of Annexin V-FITC reagent was added and gently mixed, followed by the addition of 10 L of propidium iodide staining solution, which was also gently mixed

doi: 10.1016/j.neurobiolaging.2015.05.022 416 ZhanX.StamovaB.JinL.-W.DeCarliC.PhinneyB.SharpF

Bacteriostatic Water for Injection, USP is a sterile, non-pyrogenic preparation of water for injection containing 0.9% (9 mg/mL) of benzyl alcohol added as a bacteriostatic preservative

Unlike deficiency states, which produce well-characterised neurological and haematological symptoms, there is no clearly defined clinical syndrome associated with B12 excess from supplementation alone

Required Equipment and Materials Plate reader (405 or 415 nm filter) 96-well microplate Incubator (37C) 20 l and 200 l pipettes, a multi channel pipette 5-Sulfosalicylic Acid (SSA) Solution Ethanol Assay Procedure Sulfasalazine Alters Intracellular Metabolites and Increases ROS in A549 Cells Induction of Ferroptosis by Erastin Erastin is a known inducer of ferroptosis