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The concentration of AF used to achieve this affect was well tolerated by the mice in this and previous studies [46], and it was reported previously that mammalian cells have over 95% viability when cultured in 10 M AF [68]
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AOD9604 also inhibited an enzyme called acetyl-CoA carboxylase, which is involved in making new fat
If frozen solution storage is required by a formal protocol, use controlled single-use aliquots rather than repeatedly thawing one vial
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