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The next day, the imaging buffer was replaced and, after another hour in the dark, the measurement was started (Ugalde et al ., 2022 a )

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Regarding intracellular roGFP2-based structure-function analysis of glutaredoxins, we now showed that the GSSG-dependent oxidation of the fused glutaredoxin moiety (and not the glutathionylation of the roGFP2 moiety) is rate-limiting unless saturating GSSG concentrations are present