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Cell compartment-specific, roGFPs, visualized via a fluorescence microscope or CLSM, indicate the in vivo redox state of glutathione in mitochondria, chloroplasts, the cytosol, peroxisomes (primarily GSH) and the endoplasmic reticulum (ER
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Detection at 214 nm and 280 nm confirms aromatic amino acid content Mass Spectrometry: Electrospray ionization (ESI) or MALDI provides exact molecular weight confirmation (711.9 g/mol) and sequence verification
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