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B12 Injections are a Beneficial Therapy for Chronic Hemodialysis Patients Patients with low levels of B12 who receive this vitamin via injection report an improvement in their quality of life

et al., Drug Des Devel Ther, 2015

Its level decreases with age (especially from the age of 45-50) and as the body's defence needs increase (immunity, liver detoxification, antioxidant defences...)

If the interacting protein that youre analyzing also binds to the GST only control, then this may be through non-specific interactions to the beads or to GST (Figure 8)