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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37 C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5-CGGTGCCCTGAATGAACTGC-3, reverse primer 5-CAGTCATAGCCGAATAGCCT-3) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)

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Pediatr Blood Cancer 44:264266 Schaeffer EK, West RJ, Conine SJ, Lowrey CH (2014) Multiple physical stresses induce -globin gene expression and fetal hemoglobin production in erythroid cells

Bac water means bacteriostatic water, a prepared water solution containing 0.9% benzyl alcohol

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