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Freeze-thaw cycles: if a validated frozen stock is used, make small aliquots and avoid repeated thawing
The vacuolar H + -ATPase is a multisubunit complexes that are members of the larger V-ATPase family of proton transporters and are related to the F-type ATPases exemplified by the F0F1-ATPase (complex V) of the oxidative phosphorylation process
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