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Dosage by body weight considerations Unlike many hormone-related peptides where body weight significantly affects dosing, epitalon protocols typically use fixed doses regardless of individual body mass

Serum-containing medium is where the difference should appear, with the caveat that serum IGFBP content varies between lots, so fixing the serum lot across a study is what keeps the comparison honest

Between 1% and 10% of the lysate was retained as input control, and the remaining lysate was used for immunoprecipitation

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